Biology 105
Eukaryotic Cell Biology

September 12, 2006 Optical Techniques in Cell Biology

Today's Handouts: Lecture Outline Complete Reading List (PDF)

Lecture Slides available via WebCT
(Note: Slides will be available with 24 hours of lecture)

Required Reading:

Textbook: Alberts et al (2002) Molecular Biology of the Cell pp. 547 - 578.

Koster & Klumperman (2003) Electron Microscopy in Cell Biology: integrating structure and function. Nature Reviews Molecular Cell Biology, 4: SS6–SS10 (2003) (An excellent summary of the techniques now available to correlate structure and function at the EM level).

Gaietta, Deerinck, Adams, Bouwer, Tour, Laird, Sosinsky, Tsien, & Ellisman (2002) Multicolor and Electron Microscopic Imaging of Connexin Trafficking. Science 296: 503-507. (A technical tour de force demonstrating the power of combined electron and optical imaging techniques).

Glepmans et al (2006) The fluorescent toolbox for assessing protein location and function. Science 312: 217-224. An up-to-date review of the techniques now available for protein localization using fluorescence microscopy. Excellent primer for many of the techniques that will appear in research papers this semester.

From the MBOC CD: 9.2, 9.3, 9.4: The Liver Cell, views 2, 3, and 4.

 

Newborn mice (above) two of which are transgenic mice with GFP fused to an epithelial protein.