 |
|
|
 |
 |
|
|
|
Last Updated:
Wednesday, 14-Feb-2007 08:14:55 EST
Website designed by
© 2005 Rebecca Lesiak |
|
 |
|
Department of Molecular Biology, Cell Biology, and Biochemistry |
| |
|
| |
|
| |
I am very pleased to announce that the Brown Center for Genomics and Proteomics has entered into an agreement with the Josephine Bay Paul Center at the Marine Biological Laboratory at Woods Hole which will provide DNA sequencing services to Brown and Brown-affiliated users at significantly reduced rates.
The introductory price will be $ 3.95 per reaction. The same price will be charged to all Brown and eligible hospital-affiliated laboratories (see below concerning eligibility for hospital labs). The pricing will be reviewed after the first quarter, and given sufficient demand is expected to go down.
The sequencing service will be overseen by Christoph Schorl Christoph_Schorl@brown.edu, Assistant Professor of Research in the Genomics Core of the Brown Center for Genomics and Proteomics. Christoph will be assisted by Wendy Virgadamo MCB_DNA@brown.edu, MCB Executive Assistant.
A web site has been created that will allow users to enter their sequencing requests and for Brown and MBL staff to track the flow through the system and to manage the charging of fees back to the investigators. To access the web site go to http://www.brown.edu/Departments/Molecular_Biology/facilities.html and click on the "DNA Sequencing" link. This will take you to an introductory page, where more detailed instructions can be found by using the "Help" link.
All labs who wish to use the service should contact Wendy (MCB_DNA@brown.edu) who will set up a profile on the sequencing web page for each PI. Each PI will then receive labels preprinted with codes (three letters unique to each lab followed by a string of 6 unique numbers). These will be affixed to the tubes containing the sequencing reactions, and will be used to track the samples through the system.
Since the website has restricted access, all users must have a valid Brown user ID and password. These are the same that are needed to access any other restricted Brown area, such as the electronic subscription in the library, etc. All hospital-affiliated faculty and staff who already have Brown user IDs will be able to access the system immediately. Those who do not but wish to take advantage of the sequencing service should arrange for affiliate accounts with the Brown Card office. Affiliate accounts can be arranged for anyone with a reasonable need for Brown electronic services.
For those who are currently using various off-site sequencing providers, the setup of the sequencing reactions will remain the same (or very close to same). Users will simply need to combine the template and the primer in a microfuge tube, and attach the coded label to the tube. The MBL sequencing center will do the rest.
To set up reactions, combine in a 500 ul microfuge tube: 500-1000 ng plasmid or 50-100 ng PCR product plus 30 pmol of primer. The total volume should be made up to 10 ul using distilled water. All dilutions should be made in distilled water. This information is also provided on the DNA Sequencing web site.
Once the reactions have been set up and coded, users will need to bring them to one of two collection points: the stockroom in the J.W. Wilson Laboratory (JWW), or the stockroom in the Laboratories for Molecular Medicine (LMM). The campus drop-off will shift to the new stockroom in the BioMed complex when it opens later in the fall.
At this time one sequencing run will be done per week. Reactions will be collected and shipped to MBL on each Monday, and the data will be available on Thursday afternoon or early on Friday. As demand grows we will increase the number of runs per week. The drop-off cutoff for inclusion in the shipment will be 1:00 pm at either JWW or LMM. On weeks when Monday is a holiday the shipment to MBL will go out on Tuesday.
Data will be provided to users via e-mail directly from MBL in text (FASTA) format. In addition, Christpoh Schorl will have access to the MBL ftp site and will be able to download the files with the chromatograms. These will not be provided to users on a routine basis. Users who wish to examine the chromatogram profiles for any one particular reaction should contact Christoph and he will provide the data.
Happy sequencing !!!
|
|
|
