Transgenic Facility at Brown University

Brown University
Transgenic Facility
Office of the Dean Administration Departments Research Alpert Medical School Public Health Program Giving

Welcome to the Transgenic Facility at Brown University

The function of the Transgenic Facility is to support investigators both through generation of transgenic and mutant mice. Our mission is to facilitate access to mouse models of human diseases. We are part of the Center for Genetics and Genomics at Brown University, RI. The facility is supported in part by user fees.

The green fluorescent mouse generated at Brown transgenic facility.
A single copy of EGFP is located at Egfr locus on chromosome 11
fluorescent mouse 1 fluorescent mouse 2 fluorescent mouse 3 fluorescent mouse4 fluorescent mouse 5

Mice carrying engineered chromosomal rearrangements
marked by K14-agouti transgene causing yellowing ears and tails
mice 1 mice 2

Services

  1. Targeted Mutagenesis of Embryonic Stem Cells
    Gene targeting is performed using constructs delivered by individual investigators. ES cell clones are picked up in 96 well plate format and delivered for molecular screening
  2. Generation of ES cell chimeras
    Typically, four male chimeras are generated from targeted ES cells and mated at the transgenic facility to obtain F1 animals. F1 mice are then transferred to individual investigators for further studies
  3. Transgenic mice are produced by pronuclear injections of DNA
  4. Re-derivation of mice by embryo transfer
  5. We provide expert support to guide you through all the steps in a targeted mutagenesis project

Rates

For Brown affiliated customers:

Knock-out/in animals from the vector stage  $7,000 each
Knock-out animals from delivered cells  $3,000 each
Re-derivation   $2,500
Transgenic animals  $2,000 each

For customers other than Brown affiliated faculty:

Knock-out/in animals from the vector stage  $40,000 each
Knock-out animals from delivered cells  $20,000 each
Re-derivation   $5,000
Transgenic animals  $11,000 each

Contact

For more information, contact Dr. Jan Klysik, Director
Jan_Klysik@Brown.edu
The Transgenic Mouse Lab is located in BioMed Research Building, Room 255. Tel. 401-863-9534

We encourage all investigators, particularly those from southern New England, to consider collaborative projects. In addition to transgenic, knockout and conditional knockout mice, we are equipped to engineer defined, coat color tagged segmental deletions, inversions, and translocations. Also, we can generate tetraploid blastocysts for injecting diploid ES cells. Please inquire with regard to such projects.

Selected Publications

  • Klysik. J. Singer J.D. Absence of Transmission Abnormalities in Mice with the Green Fluorescent Knocked-in Marker on Chromosome 11. (2005) Biochem. Genet. 43, 321-333.
  • Steven Theroux, Mandy Pereira, John M. Sedivy, Jan Klysik. Raf kinase inhibitory protein knockout mice: expression in the brain and olfaction deficit. (2007) Brain Research Bulletin 71, 559-567.
  • Jeffrey S Moffit, Kim Boekelheide, John M. Sedivy, Jan Klysik. Mice lacking Raf Kinase Inhibitor Protein-1 (RKIP-1) have altered sperm capacitation and reduced reproduction rates with a normal response to testicular injury (2007) J. Androl. 28, 883-890.
  • Mandy Pereira, Penelope Mason, Roman J Szczesny, Leena Maddukuri, Sylwia Dziwura, Robert Jedrzejczak, Erin Paul, Andrzej Wojcik, Lien Dybczynska, Barbara Tudek, Ewa Bartnik, Jan Klysik, Vilhelm A Bohr, Piotr P Stepien. Interaction of human SUV3 RNA/DNA helicase with BLM helicase: loss of the SUV3 gene results in mouse embryonic lethality (2007) Mechanisms of Aging and Development 128, 609-617.
  • Jan Klysik, Steven J. Theroux, John M. Sedivy, Jeffrey S. Moffit, Kim Boekelheide. Signaling Crossroads: The function of Raf Kinase Inhibitory Protein in Cancer, the Central Nervous System and Reproduction. (2008) Cellular Signalling 20,1-9.
  • Sheila A. Haley, Ting Zhao, LiJun Zou, Jan Klysik, James F. Padbury, Lazaros K. Kochilas. Forced expression of the cell cycle inhibitor p57KIP2 in cardiomyocytes protects the heart from ischemia-reperfusion injury (2008) BMC Physiology 8(1):4.
  • Erin Paul, Rachel Cronan, Paula J. Weston, Kim Boekelheide, John M.Sedivy, Sang-Yun Lee, David L. Wiest, Murray B. Resnick, Jan E. Klysik. Disruption of Supv3L1 damages the skin and causes sarcopenia, loss of fat, and death (2009) Mamm Genome 20, 92-108.
  • Aron Gyuris, Diana J Donovan, Kimberly A Seymour, Lindsay A Lovasco, Nathaniel R Smilowitz, Anthony L Halperin, Jan E Klysik, Richard N Freiman. The Chromatin Targeting Protein Brd2 is Required for Neural Tube Closure and Embryogenesis (2009) Biochim Biophys Acta 1789,413-421.
  • Barbara Giovannone, William G. Tsiaras, Suzanne de la Monte, Jan Klysik, Corinne Lautier, Galina Karashchuk, Stefano Goldwurm, and Robert J. Smith. GIGYF2 Gene Disruption in Mice Results in Neurodegeneration and Altered Insulin-like Growth Factor Signaling. (2009) Human Molecular Genetics 18(23), 4629-39.
  • Caffery P, McCann CM, Tapia JC, Hartlaub H, Klysik J, Cooper E, Lichtman JW, Hawrot E. Engineering neuronal nicotinic acetylcholine receptors with functional sensitivity to alpha-bungarotoxin: A novel alpha3-knock-in mouse. (2009) European Journal of Neuroscience DOI:10.1111/j.1460-9568.2009.07016.x
  • Erin Paul, Marissa Kielbasinski, John M. Sedivy, Carlos Murga-Zamalloa, Hemant Khanna, Jan E. Klysik. Widespread expression of the Supv3L1 mitochondrial RNA helicase in the mouse. (2010) Transgenic Research DOI: 10.1007/s11248-009-9346-0