Next Generation Sequencing allows massively parallel sequencing of DNA to obtain millions of reads of DNA sequences, supporting applications such as de novo sequencing, gene expression analysis, ChIP-sequencing and re-sequencing. One of the powerful advantages of Next Generation Sequencing is that it is not limited to species with known reference genomes. In fact, our Facility has successfully sequenced samples derived from a wide range of species including spinach, deep sea invertebrates, Drosophila, mouse and human.
The HiSeq2000 employs the same sequencing technology as the GAIIx with the advantage that 2 flowcells can be run simultaneously independent of the run type. Currently we run 50 - 100 bp single read and paired-end read. Illumina's ongoing improvement of sequencing technology will soon enable reads of up 150 bp. Due to different imaging capabilities the run length is reduced compared to the GAIIx and due to higher capacity of the flowcell each run yields about 2-fold more data compared to the GAIIx. With its increased data output capacity, the HiSeq is highly suited to clinical sequencing projects. We routinely obtain 150 - 200 million reads per lane on the HiSeq, yielding up to 300 GB of data per flow cell, depending on read length and library quality.