HUA's Protocol

HUA's Protocol

• Chromatin Immunoprecipitation Protocol (ChIP)
• Hemolymph collection from adult Drosophila
• Hemolymph insulin-like peptide (DILP) measurement by ELISA
• Ecdysteroid titers estimation by EIA
• Vitellogenin protein estimation by ELISA
• Triglycerides Measurement

• PCR
• RNA isolation
• dsRNA synthesis
• Quick Genomic DNA Isolation
• Paraffin section
• p-Histone staining
• TUNEL
• Whole-mount In Situ Hybridization