The COBRE CCRD core facility provides state-of-the-art instrumentation, support, and technical training for the Department of Pathology and Laboratory Medicine and faculty at the Warren Alpert Medical School and its affiliated hospitals. Services include automated immunohistochemical staining, tissue microarry production, laser capture microdissection, quantitative real-time PCR, and data and image analysis.
This core facility, located at the Laboratories for Molecular Medicine at 70 Ship Street, provides scientific evaluation and diagnosis of pathologic alterations at both the cellular and organismal level for animal models of toxicant-induced disease. The following services are provided: paraffin, plastic, and frozen tissue sample preparation for light microscopy, biological sample preparation for transmission and scanning electron microscopy, and whole mouse embryo embedding and serial sectioning.
Lifespan Core Research Laboratories offers a broad spectrum of technical services to the research community on a fee-for-service basis. The digital imaging and histology departments are located in Coro West Building, 1 Hoppin St, 4th floor, rooms 4.211 and 4.213, respectively. The electron microscopy department is located in the basement of Grad's Dorm (which is located between the POB and Plain St.) and serves as a drop-off point for histology samples.
The bank provides fresh and frozen human tumor and normal tissue samples for research purposes. The tissue samples represent a cross section of specimens, including colon, stomach, lung, breast, thyroid, kidney, pancreas, and a variety of other organs surgically resected at Rhode Island Hospital and Women and Infants Hospital. Typically, paired tumor and normal tissue samples are banked from each of the available specimens. Samples are snap frozen in liquid nitrogen and stored at -80°C. Details about the pathologic diagnosis for each sample are also provided upon request. All requests for samples are evaluated by a committee of three physicians, and approvals are based strictly on the merits of the research. This tumor bank is supported by the COBRE Center for Cancer Research Development and the George Endowment at Lifespan / Rhode Island Hospital.
Women and Infants Hospital is the major referral center for all high-risk pregnancies (21,000 deliveries annually) in Southeastern New England and a major participant in prenatal screening programs. This hospital has an extensive archival collection of maternal and fetal serum samples, cytogenic analyses, placental samples, and perinatal autopsies available for clinical and translational research. Dr. M. Halit Pinar is Director of the Division of Perinatal Pathology and oversees this tissue and specimen bank at Women and Infants Hospital.
The Tissue Resource Center at Brown performs neuropathologic examinations on all brain and tissue donations from Alpert Medical School's affiliated hospitals and distributes these tissues to investigators engaged in research. Neuropathologic examination procedures maximize the information obtained from individual brains and optimize the quality of the tissues distributed to specific research projects. Standardized protocols for brain dissection and blood and cerebral spinal fluid (CSF) collection ensure an identical and complete evaluation of all cases. Widely accepted diagnostic criteria are used to permit precise pathologic classification in accordance with current standards utilized at other academic and medical centers.
These tissue banks are anonymous non-profit programs in accordance with HIPAA and Institutional Review Board (IRB) regulations. Investigators are required to complete a detailed tissue request and IRB project approval.
Resources for Researchers
CoresRI.org, is an online directory of shared research instrumentation and services at Brown and other academic and medical institutions in Rhode Island.