Dr Jay's Lab: Musculoskeletal Trauma Lab
History
The Department of Emergency Medicine has an established laboratory located on the Rhode Island Hospital campus in CORO West, Suite 4.303. This laboratory is staffed by investigators from the Department of Emergency Medicine and the Brown University Division of Engineering. Research activities are focused on Bio-Engineering and Biophotonics Projects which are translationally important to the care of Emergency Medicine patients.
Investigators and Collaborators
Gregory D. Jay, MD, PhD
Dr. Jay is a professor in the Department of Emergency Medicine and Division of Engineering at Brown University. He also serves as an adjunct professor in the Department of Biomedical Sciences at the University of Rhode Island. In addition to his academic activities, he brings almost 25 years of experience as an emergency medicine physician, and he is currently employed in that capacity by University Emergency Medicine Foundation, practicing at Rhode Island Hospital and Miriam Hospital in Providence, Rhode Island. Dr. Jay, who holds an MD, PhD (in experimental pathology) and double bachelor's degrees (in engineering and biochemistry) from State University of New York at Stony Brook, is a member of numerous professional societies and is a journal reviewer for several journals in the fields of engineering, emergency medicine, and several other medical specialties. He holds over a dozen issued patents and pending patent applications covering diverse technologies in pulsus paradoxus monitoring and the lubrication of mammalian joints. He has published 140+ articles in medical and scientific journals and is a recipient of support from NIH. He is co-editor of "Liquid Crystals: Frontiers in Biomedical Applications" (World Scientific Press, 2007). Dr. Jay is also a scientific co-founder of Lµbris, LLC (www.lubris.net) based in Framingham, MA. This startup company is developing biotribological treatments for dry eyes, prevention of posttraumatic osteoarthritis, surgical adhesion prevention and other unmet medical needs. The technology central to the company’s growth after signing a licensing agreement with Novartis is the production of recombinant human PRG4 (lubricin) based on intellectual property and patents awarded to Dr. Jay and licensed to Lµbris.
Ling Zhang, MD
Dr. Ling Zhang is a senior research scientist in the Department of Emergency Medicine, Rhode Island Hospital. She received her MD from Henan Medical University in China. She worked as a research associate and was trained as a molecular and cellular scientist in the Department of Pharmacology and Toxicology at the Virginia Commonwealth University. Dr. Zhang was then appointed as a specialist to study immunology and toxicology. Prior to joining the Department of Emergency Medicine, she was the laboratory manager and senior research scientist investigating molecular mechanism(s) of inter-alpha inhibitor proteins in sepsis in the Department of Medicine at Rhode Island Hospital. Currently, Dr. Zhang is working in Dr. Jay's laboratory studying the molecular signaling pathway of Lubricin in mediating chondroprotection of diarthrodial joints. She has published 20 articles in medical and biological journals. Her primary expertise includes: cell and tissue culture, bone marrow culture, monoclonal antibody production, immunoblotting, ELISA, protein purification, molecular cloning, mutagenesis, siRNA, RNase protection assay, immunostaining, histology, flow cytometry, animal surgery, breeding and genotyping.
What's Happening in the Lab?
Research Developments in the Laboratory
Recent Notable Publications
Iqbal SM, Leonard C, Regmi S, De Rantere D, Tailor P, Ren G, Ishida H, SJ. Pang D, Salo P, Vogel HJ, Hart DA, Waterhouse CC, Jay GD, Schmidt TA, Krawetz RJ. Lubricin/Proteoglycan 4 binds to and regulates the activity of Toll-Like Receptors In Vitro. Scientific Reports. 2016 Jan 11;6:118910. [PMID: 26752378] [PMCID: PMC4707532]
Karamchedu NP, Tofte JN, Waller KA, Zhang LX, Patel TK and Jay GD. Superficial zone cellularity is deficient in mice lacking lubricin: A stereoscopic analysis. Arthritis Research and Therapy. 2016 Mar 14;18(1):64. [PMID: 26975998]
Torres, JR., Jay, GD., Kim, KS, Bothun, GD. Adhesion in Hydrogel Contacts. Royal Society. Published 18 May 2016.DOI: 10.1098/rspa.2015.0892
Zhao C, Ozasa Y, Shimura H, Reisdorf R, Thoreson A, Jay G, Moran S, An K, Amado PC. Effects of Lubricant and Autologous bone marrow stromal cell augmentation on Immobilized Flexor Tendon Repairs. Journal of Orthopaedic Research. 2016; 34(1):154-60. [PMID: 26177854]
Larson KM, Zhang, L, Elsaid KA, Schmidt TA, Fleming BC, Badger GJ, Jay GD. Reduction of friction by recombinant human proteoglycan 4 in IL-1α stimulated bovine cartilage explants. Journal of Orthopedic Research. 2016 Jul 13. doi: 10.1002/jor.23367. [Epub ahead of print] [PMID: 27411036]
Teeple E, Karamchedu NP, Larson K, Zhang L, Badger GJ, Fleming BC, Jay, GD. Arthroscopic Irrigation of the Bovine Stifle Joint Increases Cartilage Surface Friction and Decreases Superficial Zone Lubricin. Journal of Biomechanics. 2016 Jul 27. pii: S0021-9290(16)30800-4. doi: 10.1016/j.jbiomech.2016.07.024. [Epub ahead of print] [PMID: 27511596]
Choi B, Asselin N, Pettit CC, Dannecker M, Machan JT, Merck DL, Merck LH, Suner S, Williams KA, Jay GD,Kobayashi L. Simulation-based Randomized Comparative Assessment of Out-of-Hospital Cardiac Arrest Resuscitation Bundle Completion by Emergency Medical Service Teams using Standard Life Support or an Experimental Automation-Assisted Approach. Simulation in Healthcare. 2016 Aug 9. [Epub ahead of print] [PMID: 27509064]
Reesink H, Watts AE, Mohammed HO, Jay GD, Nixon AJ. Lubricin/Proteoglycan 4 Increases in both Experimental and Naturally Occurring Equine Osteoarthritis. Osteoarthritis and Cartilage. 2016 Aug 4. pii: S1063-4584(16)30214-X. doi: 10.1016/j.joca.2016.07.021.
Zhang M, Mani SB, He Y, Hall AM, Xu L, Li Y, Zurakowski D, Jay GD, Warman ML. Induced superficial chondrocyte death reduces catabolic cartilage damage in murine posttraumatic osteoarthritis. Journal of Clinical Investigation. 2016 Aug 1;126(8):2893-902. [PMID: 27427985] [PMCID: PMC4966316]
Lambiase A, Sullivan BD, Schmidt TA, Sullivan DA, Jay GD, Truitt ER, Bruscolini A, Sacchetti M, Mantelli F. A two week, randomized, double-masked, study to evaluate safety and efficacy of Lubricin (150ug/mL) eye drops versus sodium hyaluronate (HA) 0.18% eye drops (Vismed®) in patients with moderate dry eye. Ocular Surface. DOI: http://dx.doi.org/10.1016/j.jtos.2016.08.004
Oh J, Kuan KG, Tiong LU, Trochsler MI, Jay G, Schmidt TA, Barnett H, Maddern GJ. Recombinant human lubricin for prevention of postoperative intra-abdominal adhesions in a rat model. Journal of Surgical Research. 2017 Feb;(208):20-25.
Waller KA., Chin KE., Jay GD., Zhang LX., Teeple E., McAllister S., Badger GD., Schmidt TA., Fleming BD. Intra-articular rhPRG4 mitigates cartilage damage after destabilization of the medial meniscus in the Yucatan minipig. American Journal of Sports Medicine. 2017. doi: 10.1177/0363546516686965
Zhang XC., Bermudez AM., Reddy PM., Sarpatwari RR., Chheng DB, Mezoian TJ., Schwartz VR., Simmons QJ., Jay GD., Kobayashi L. Interdisciplinary development of an improved emergency department procedural work surface through iterative design and use testing in simulated and clinical environments. Annals of Emergency Medicine. 2016 Nov 14. pii: S0196-0644(16)30914-3. doi: 10.1016/j.annemergmed.2016.08.436
Grants:
Non-tribologic Bioactivity of Lubricin. National Institutes of Health National Institute of Arthritis and Musculoskeletal and Skin Diseases, 1R01AR067748. Principal Investigator. $2,190,726 awarded 5/10/16 – 4/30/2021.
Use of Multifunctional rhPRG4 Biologic for Treatment of Traumatic Brain Injuries. National Institute of Neurological Disorders and Stroke, 1R21NS099605-01. Co-Investigator. $429,330 awarded. 9/30/2016-8/31/2018.