Due to the high pathogenicity of the virus responsible for the Covid-19 pandemic and the lack of an effective treatment, live SARS-CoV-2 viruses must be handled under Biosafety Level 3 (BSL-3) conditions, which has hindered the development of vaccines and therapeutics.
The Lentivirus Construct Core of the COBRE for Stem Cells and Aging has developed a pseudotyped SARS-CoV-2 virus which has a lentiviral core but with the SARS-CoV-2 spike protein on its envelope.
The pseudoviruses infect human lung epithelial cells in an ACE2-dependent manner and confer the expression of a fluorescence protein ZsGreen in infected cells for imaging and quantification. The pseudoviruses can only accomplish a single infection cycle and are replication incompetent, thus require only BSL-2 level containment.
- Pseudoviruses bearing SARS-CoV-2 D614 spike (earlier prevalent strain)
- Pseudoviruses bearing SARS-CoV-2 G614 spike (currently dominant strain)
- Pseudoviruses bearing SARS-CoV-2 R685A spike (furin-cleavage site mutated)
Service fees apply. Contact Olin Liang, PhD to get started.