COBRE for Stem Cells and Aging

COBRE for Stem Cells and Aging: Lentivirus Construct Core

Multiple SARS-CoV-2 variants are circulating globally since the COVID-19 pandemic started in early 2020. Most notable strains include D614G, the U.K. (B.1.1.7), South Africa (B.1.351) and Brazil (P.1) variants. Much of the research effort is focused on the mutations in the spike (S) protein that locks onto a human protein ACE2 on the cells to cause infection.

At the Lentivirus Construct Core, we have developed a pseudotyped SARS-CoV-2 virus which has a lentiviral core but with the SARS-CoV-2 spike protein on its envelope (Zhou et al., Oncotarget, 2020, 11(46): 4201-4223). The pseudoviruses infect human lung epithelial cells in an ACE2-dependent manner and confer the expression of both luciferase and a fluorescence protein ZsGreen in infected cells for imaging and quantification. The pseudoviruses can only accomplish a single infection cycle and are replication incompetent, thus require only BSL-2 level containment.

Available to Investigators (Fees Apply):

  • Pseudovirus with SARS-CoV-2 N501Y spike (a common mutation in the B.1.1.7, B.1.351 and P.1 variants in the winter of 2020/2021)
  • Pseudovirus with SARS-CoV-2 E484K spike (in the B.1.351 and P.1 variants)
  • Pseudovirus with SARS-CoV-2 N501Y+E484K spike (in the B.1.351 and P.1 variants)
  • Pseudovirus with SARS-CoV-2 N501Y+E484K+K417N spike (in the B.1.351 and P.1 variants)
  • Pseudovirus with SARS-CoV-2 G614 spike (dominant strain since the spring of 2020)
  • Pseudovirus with SARS-CoV-2 D614 spike (prevalent strain in early 2020)
  • Pseudovirus with SARS-CoV-2 R685A spike (furin-cleavage site mutated)
  • Lentivirus for S protein expression with its original gene sequence
  • Lentivirus for S protein over-expression with a codon-optimized gene sequence
  • Lentivirus for human ACE2 over-expression with puromycin or blasticidin selection
  • Stable HeLa cell line over-expressing hACE2 (highly efficient in pseudovirus cell entry)

Contact: Olin Liang, PhD
Director, Lentivirus Construct Core
Tel: 401-444-9039 (work), 617-816-8885 (cell) | Email: [email protected]