FACSAriaIIIu Flow Cytometer and Cell Sorter

The BD FACSAria is a sorter that incorporates a fixed-alignment cuvette flow cell. This new flow cell provides superior fluorescence sensitivity. The FACSAria has four air-cooled lasers at 488nm, 561nm, 633nm, and 407nm allowing multicolor analysis of up to 19 parameters. The FACSAria can sort 1, 2, 3, or 4 populations simultaneously and is equipped with an Automated Cell Deposition Unit for sorting into multiwell plates or slides. The FACSAria is housed in a Baker Bioprotect E3 hood with an Aerosol Management System that evacuates any aerosols from the sort chamber, providing an additional level of safety for the operator while maintaining sterility for samples. The FACSAriaIIIu also has a refrigeration system that allows cooling of the sort sample as well as the sort collection tubes. It can accept various sample input tubes including 1 mL microtubes, 12 x 75-mm test tubes, and 15 mL tubes into the sample and collection chambers.

This facility was supported in part by NCCR equipment grant 1S10RR021051, the Division of Biology and Medicine and the Provost's office.