FACSAriaIIIu Flow Cytometer and Cell Sorter

The BD FACSAriaIIIu is a sorter that incorporates a fixed-alignment cuvette flow cell. This new flow cell provides superior fluorescence sensitivity. The FACSAriaIIIu has fice air-cooled lasers at 355nm, 407nm, 488nm, 561nm, and 633nm allowing for multicolor analysis of up to 20  parameters. The FACSAria can sort 1, 2, 3, or 4 populations simultaneously and is equipped with an Automated Cell Deposition Unit for sorting into multiwell plates or slides. The FACSAriaIIIu is housed in a Baker Bioprotect E3 hood with an Aerosol Management System that evacuates any aerosols from the sort chamber, providing an additional level of safety for the operator while maintaining sterility for samples. The FACSAriaIIIu also has a refrigeration system that allows cooling of the sort sample as well as the sort collection tubes. It can accept various sample input tubes including 1 mL microtubes, 12 x 75-mm test tubes, and 15 mL tubes into the sample and collection chambers.

This facility was supported in part by NCCR equipment grant 1S10RR021051, the Division of Biology and Medicine and the Provost's office.