- q-PCR - ViiA 7 Real Time PCR Systems
- ddPCR - Bio-Rad QX200 Droplet Digital PCR (ddPCR) System
- Infrared Imaging System - LiCor Odyssey Clx
- NEW! Sprint Profiler - NanoString - nCounter Sprint Profiler
- Plate Reader Cytation™ 5 Cell Imaging Multi-Mode Reader
- Multi-Mode Reader - Synergy H1 Hybrid Multi-Mode Reader
- Spectrophotometer - Thermo Scientific NanoDrop One
- Ultracentrifuge - Beckman Coulter Optima Max
- Cell Counter - Life Technologies Automated Countess Cell Counter
- Microarray Scanner - Molecular Devices GenePix Scanner 4000B
- Sequencer - Illumina NextSeq550 High Throughput Benchtop Sequencer
- Microarray Platform - Affymetrix GeneChip Instrument System
- Agilent Bioanalyzer 2100
- Ultrasonicator - Covaris S220
- Flow Cytometry - Becton Dickinson (BD) Biosciences FACSMelody
Affymetrix GeneChip Instrument System
The Affymetrix GeneChip system consists of a gene or probe array, hybridization oven, fluidics station, scanner, and a computer workstation. For more details please see the "Services" page.
Use of the Affymetrix equipment is limited to the Genomics and Proteomics Laboratory Manager.
Agilent Bioanalyzer 2100
The Genomics Core Facility offers quality/integrity analysis for RNA and DNA samples. Please see "Services" for more information.
Real Time qPCR / PCR Thermal Cycler
Applied Biosystems ViiA 7 Real Time System
Two ViiA 7 Real Time Systems are available for use. The ViiA 7 systems use fluorescent-based PCR to provide quantitative detection of nucleic acid sequences using real-time analysis and qualitative detection of nucleic acid sequences using end-point and melting curve analysis. They can be used with TaqMan probe-based chemistry or intercalating dyes such as SYBR Green. The ViiA 7 qPCR machines can be run with a 96, 384 and TLDA block configuration.
Droplet Digital PCR System
Bio-Rad QX200 Droplet Digital PCR (ddPCR) System
The QX200 Droplet Digital PCR (ddPCR™) System provides absolute quantification of target DNA or RNA molecules for EvaGreen or probe-based digital PCR applications.
Beckman Coulter Optima Max Ultracentrifuge
The Optima Max Ultracentrifuge is capable of handling volumes from just a few µl up to 6.4 ml. Applications include differential gradient separations of proteins, DNA and RNA, lipoproteins, subcellular fractionation, membrane fractionation and sedimentation of viruses. Both fixed angle and swing bucket rotors are available. The following rotors are available for use: TLA100, TLA110, MLA130 (all fixed angle), TLS55 (swing bucket).
Flow Cytometry / Fluorescence Activated Cell Sorter (FACS)
Becton Dickinson (BD) Biosciences FACSMelody Flow Cytometer
The BD FACSMelosy Flow Cytometer is frequently used for cell cycle analysis, protein expression, and measurement of metabolic activity. The FACSCalibur has one laser with an excitation line at 488 nm, allowing three color fluorescence analysis. Emission signals are centered at 530 nm, 585 nm, and 682 nm. The BD FACSChorus software is used to operate the instrument, acquire and sort samples, and analyze the data.
Fluorescent Western Scanning / Imaging System
LiCor Odyssey Clx Infrared Imaging System
The Odyssey Infrared Imaging System allows multiplex Western Blotting and accurate protein detection and quantification using secondary antibodies labeled with IRDye near-infrared (NIR) fluorescent dyes.
Life Technologies Automated Countess Cell Counter
The Invitrogen Countess provides quick determination of viability and concentration of cells in suspension derived from tissue culture and/or isolated from tissue. It is a convenient alternative to performing time consuming cell counts with a hemocytometer. The Countess uses trypan blue exclusion to distinguish between dead and living cells. Users are charged for slides used.
Please contact Christoph Schorl to discuss biological safety prior to use.
Scanning of Glass Slide-Based Arrays / Microarray Scanner
AXON Molecular Devices GenePix4000B Scanner
The Axon 4000B instrument scans individual slides in 2 colors (cy3 & cy5) using Genepix Software and simultaneously scans arrays on glass slides at two wavelengths using a dual laser scanning system, which dramatically reduces scan time.
The Core Facility also has the equipment required for hybridization of glass slide based arrays.
Cell Imaging Multi-Mode Reader
Synergy H1 Hybrid Multi-Mode Reader
Synergy™ H1 is a configurable multi-mode microplate reader. Choose monochromator-based optics for flexibility, filter-based optics for sensitivity, or both…BioTek’s patented Hybrid Technology™ offers high-performance and applications versatility in a modular platform to expand as your laboratory’s needs change. Top and bottom fluorescence intensity, UV-visible absorbance and luminescence detection are available to meet many life science research requirements.
Cell Imaging Multi-Mode Reader
BioTek Cytation™ 5 Cell Imaging Multi-Mode Reader
Cytation™ 5 combines automated digital microscopy and conventional microplate detection in a configurable, upgradable platform. The microscopy module offers up to 60x magnification in fluorescence, brightfield, high contrast brightfield, color brightfield and phase contrast for maximum applications reach. The multi-mode detection modules include filter- and monochromator-based fluorescence detection, luminescence and UV-Vis absorbance detection. Gen5™ software provides complete control over all imaging and data capture, plus powerful image and data analysis.
Thermo Scientific NanoDrop One
The NanoDrop One measure absorbance and is an alternative to a traditional cuvette spectrophotometer and allows the convenient measurement of small volumes (approximately 1.5 ul) to determine accurately RNA and DNA concentrations. The software displays the concentration in ng/ul together with some QC metrix.
The use of the Core Facility Nanodrop is restricted to nucleic acid measurement.
This system is a high throughput benchtop sequencer that provides accurate fast sequencing data, and by employing flexible run configurations it can be easily adopted to a researcher’s needs depending on their required read length and sequencing depth.
DNA & RNA Analysis
NanoString - nCounter Sprint Profiler
Molecular barcoding technology for RNA, DNA, and protein profiling applications. Digitally examine multiple pathways in a single tube in an extraction-free workflow. Included nSolver Analysis Software. Compatible with FFPE tissue. Highly multiplexed analysis of many functional pathways such as among others cancer, inflammation, cell death and custom designed expression analysis. Operated as fee for service.
Covaris S220 Focused-ultrasonicator
The S220 Focused-ultrasonicator is a versatile, high power system engineered for pre-analytical sample processing using Covaris’ patented Adaptive Focused Acoustics® (AFA®). Utilizing a spherical acoustic transducer, specifically designed acoustical cuvettes and advanced electronics, AFA delivers controlled energy precisely and accurately to sample volumes from 125 μl (microtube) to 200 ul (minitube). The Covaris S220 can be used for processing of RNA, Protein and DNA. The precisely controlled delivery of energy is less damaging to DNA than that of tradional sonicators and it also allows to shear DNA to a user-defined and uniform size in a reproducible manner.