Nucleic Acids Analysis with Agilent Bioanalyzer

Nucleic Acids Analysis with Agilent Bioanalyzer

The Core Facility staff offers analysis of nucleic acid samples (both RNA and DNA) using the Agilent 2100 Bioanalyzer. This instrument provides an objective measure of nucleic acid quality that is more informative than traditional agarose gel results, is faster, and eliminates the use of ethidium bromide. The bioanalyzer chip uses microfluidics technology and voltage induced size separation in gel-filled chambers detecting nucleic acids with laser induced fluorescence. The resulting electropherogram trace is converted into a gel like image. For RNA, the Agilent software computes a RNA Integrity Number (RIN) using a number of features from the electropherogram, such as the ratio of 28S to 18S ribosomal RNA. This value provides a qualitative assessment of the RNA. DNA assays on the bioanalyzer can be used to assess the size and purity of DNA for downstream applications such as cloning or NextGen sequencing.

The user provides samples at the required concentration and Core Facility staff will run the samples on the bioanalyzer chip and email the results to the user in an easily viewable PDF file format. Analysis can usually be arranged within 2 business days. Please contact the facility staff for specifics on sample preparation.