Reserve an Instrument

ATTENTION! NEW Phase 2 regulations

Only researchers approved for resuming research stage 1 or stage 2 can enter the Genomics Core and perform experiments in the Genomics Core.

Two persons apart from the Core Director are allowed in the Genomics Core, but they cannot work in the same bay.

Please indicate in the room calendar which instrument you will be using, e.g. qPCR or plate reader.

 All other rules from Phase 1 still apply.

Prior to reserving an instrument, please reserve the room in the Genomics Core Facility Room 109 calendar.  

 

Room Reservation Instructions: Genomics Core Facility Room 109

1. Open your Google Calendar through your Brown Gmail account.
2. Create an event by either:
     a. clicking the "Create" button on the top left of your calendar
     or
     b. clicking on the area in the calendar (Date and Time) that you want to reserve.
3. Add the event name and ensure the correct date and time.
4. Copy and paste the following Google Calendar ID into the "Add Guests" field.
Calendar ID: [email protected]
5. Click the save button once you are finished. 

 

Instrument Reservation:

Internal users can click on the reservation button below to reserve the following instruments:

  • ViiA 7 Real Time PCR Systems  [A]
    96 well block
    TLDA block
    384-well block
  • ViiA 7 Real Time PCR Systems [B]
    96/TLDA block
    384-well block 
  • BIO-RAD QX200 Digital Droplet PCR
  • FACSMelody
  • Beckman Ultracentrifuge
  • Li-Cor Odyssey System
  • Countess Cell Counter
  • Genepix Scanner
  • Nanodrop One
  • Cytation 5 Plate Readers
  • Synergy H1 Hybrid Multi-Mode Reader

 Image result for reserve buttonhttp://www.brown.edu/Research/CGP/core/equipment/calendar/index.php.

For all other instruments and services, please contact the Facility Manager.

External users may reserve equipment by contacting Christoph Schorl by email at [email protected] or by phone at (401) 863-2875.

Following are the instruments available at the genomics Core Facility

Instruments available - FY19

Instrument Manufacture Service
Bioanalyzer Agilent  Bioanalyzer 2100

Microfluidics-based platform for sizing, quantification and quality control of DNA, RNA, proteins and cells

Capillary Electrophoresis Advanced Analytical, Fragment Analyzer

Analyze DNA fragments for Next-Gen Sequencing, genotyping, checking RNA quality and quantity, and working with microsatellite simple sequence repeats (SSRs).

Cell Counter Life Technologies Automated Countess Cell Counter

Measure cell count and viability (live, dead, and total cells)  using the standard trypan blue technique

Centrifuge Sorvall Legend T Benchtop

Low to medium speed centrifugation

Centrifuge - Ultra Beckman Coulter Optima Max 

High speed centrifugation with MLA130, TLA110 & TLA100 Rotors

Disrupter Covaris S220 High Performance Ultrasonicator

DNA, RNA, and chromatin shearing, tissue homogenization, cell lysis, compound dissolution, and particle micronization

Fluorescence Activated Cell Sorted (FACS) Becton Dickinson (BD) Biosciences FACS Calibur 

Flow cytometry cell analysis and cell sorting

Hybridization Oven Agilent  G2534A 

Microarray hybridization oven

Imaging System LiCor Odyssey Clx Infrared

Western blot analysis, EMSA, protein arrays, In-Cell Western Assays, On-Cell Westerns, in vivo imaging, Coomassie gel documentation, DNA gel documentation, and tissue section analysis. 

Microarray Platform Affymetrix Gene Chip  Instrument System

A fully integrated platform for Affymetrix microarrays

Microarray Scanner Molecular Devices GenePix Scanner 4000B

Two color microarray scanner

QX200™ Droplet Digital PCR System 
BIO-RAD Droplet Digital PCR System 

The QX200 Droplet Digital PCR (ddPCR™) System provides absolute quantification of target DNA or RNA molecules for EvaGreen or probe-based digital PCR applications.)

Microplate Reader BioTek Cytation 3 Cell Imaging Multi-Mode Reader UV-Vis absorbance, Fluorescence intensity, Luminescence, Fluorescence polarization, Time-resolved fluorescence
PCR Thermal Cycler Applied Biosystems viia-7-real-time-system

Medium to high throughput 96- and 384-well fast real time quantitative PCR formats, medium to high throughput

PCR Thermal Cycler Applied Biosystems viia-7-real-time-system

Medium to high throughput 96- and 384-well fast real time quantitative PCR formats, medium to high throughput

PCR Thermal Cycler Eppendorf  Gradient Mastercycler 

Gradient cycler enables the temperature across the entire block to be varied within a range of up to 20 C; it can be programmed with every temperature step. For use by genomics facility personnel.

Spectrophotometer Thermo Scientific NanoDrop One

A  microvolume spectrophotometer for measuring DNA, RNA, and protein.

Technical Assistance- Affymetric Microarray Service

Service to provide Affymetrix gene expression array service

NGS library quality control Service

Analysis of library size distribution and qPCR quantification