TRANSGENIC FACILITY INTERNAL PRICING*                                                                                      (Fiscal Year 2020)

CRISPR/ Cas9 Services

CRISPR  - Project Design and Reagent Preparation $1,000
CRISPR - Guide RNA validation
CRISPR - Founder Genotyping (Standard) $10/sample
CRISPR - Germline Transmission $1,621

Donor Construction/Preparation

 Knock-In (KI, >80bp) $1,354
Conditional Knock-Out (cKO - two steps) $400
Point Mutation (PM) $200
* to be combined with 'Embryo microinjection/Electroporation' service for mouse model generation

Embryo Microinjection/Electroporation 

Embryo Microinjection/Electroporation (B6) $4,857
Embryo Microinjection/Electroporation (FVB) $3,995
Embryo Microinjection/Electroporation (CD-1) $2,968

Cryopreservation Services

Embryo Cryopreservation (Homozygotes) $1,000
Embryo Cryopreservation (Heterozygotes) $1,200
Sperm Cryopreservation $804
Cryo Recovery - From Frozen Embryo
Cryo Recovery - From Frozen Sperm

ES Cell Gene Targeting

Electroporation $1,907
Clone Selection $1,935
Scale Up Archive Clones $651
Microinjection of 129 ES Cells $3,815
Microinjection of B6 ES Cells $5,995
De Novo generation of ESC lines from existing transgenic line $2,911
Custom ES Injection (mice from PI) $3,030

In Vitro Fertilization (IVF)

IVF - Fresh sperm $1,451
IVF - Frozen sperm $1,958
IVF plus Sperm Cryo $1,853
IVF plus Embryo Cryo $1,963

Other Services

Random Integration Transgenesis $5,435
Rederivation $1,218
Colony Scale Up $2,500
Germline Transmission
Genotyping $10/Sample
Housing $1.22/Diem
Annual Cryo Storage Fee $162/Year
Hourly Rate for Custom Projects Not Listed $68/Hour

*Rates for Brown and Rhode Island Academic and Hospital Affiliates. For External Academic or Commercial Rates, contact  Jinping Luo ([email protected]

Rates Effective 10/1/2019