Publications

Google Scholar Citations Link - for a full listing of papers and published abstracts.

*Bolded names indicate graduate students in the Darling Lab.


2016

Beane OS, Fonseca VC, Darling LEO, Darling EM. (2016) Disparate response to methotrexate in stem versus non-stem cells. Stem Cell Rev Reports. NIHMSID: 755331DOI:10.1007/s12015-016-9645-9

López-Fagundo C, Livi LL, Ramchal T, Darling EM*, Hoffman-Kim D*. (2016) A biomimetic synthetic feeder layer supports the proliferation and self-renewal of mouse embryonic stem cells. Acta Biomater. NIHMSID: 789354. *Co-corresponding author. DOI:10.1016/j.actbio.2016.04.047

Chen Y, Cossman J, Jayasuriya CT, Li X, Guan Y, Fonseca VC, Yang K, Charbonneau C, Yu H, Kanbe K, Ma P, Darling EM, Chen Q. (2016) Deficient Mechanical Activation of Anabolic Transcripts and Post-Traumatic 1 Cartilage Degeneration in Matrilin-1 Knockout Mice. PLoS ONE DOI:10.1371/journal.pone.0156676


2015

Dingle YL, Chirila AM, Boutin ME, Livi LL, Labriola NR, Jakubek LM, Morgan JR, Darling EM, Kauer JA, Hoffman-Kim D. (2015) Three-Dimensional Neural Spheroid Culture: An In Vitro Model for Cortical Studies. Tissue Eng C. PMCID: PMC4663656DOI:10.1089/ten.tec.2015.0135 

Darling EM, DiCarlo D. 2015. High-throughput assessment of cellular mechanical properties. Ann Rev Biomed Eng. PMID: 26194428DOI:10.1146/annurev-bioeng-071114-040545

Labriola NR, Darling EM. 2015. Temporal heterogeneity in single-cell gene expression and mechanical properties during adipogenic differentiation. J Biomech. 28(2015): 1058-66. PMCID: PMC4380682DOI:10.1016/j.jbiomech.2015.01.033


2014

Marble HD, Sutermaster BA, Kanthilal M, Fonseca VC, Darling EM. (2014). Gene expression-based enrichment of live cells from adipose tissue produces subpopulations with improved osteogenic potential. Stem Cell Res Ther. 5(5):145. PMCID: PMC4619280DOI:10.1186/scrt502

Beane OS, Fonseca VC, Cooper LL, Koren G, Darling EM. (2014). Impact of aging on the regenerative preoperties of bone marrow-, muscle-, and adipose-derived mesenchymal stem/stromal cells. PLoS ONE. 9(12): e115963. PMCID: PMC4277426DOI:10.1371/journal.pone.0115963

Kanthilal M, Darling EM. (2014). Characterization of mechanical and regenerative properties of human, adipose stromal cells. Cell Mol Bioeng. NIHMSID: 618972. DOI: 10.1007/s12195-014-0350-y 

Toyjanova J, Hannen E, Bar-Kochba E, Darling EM, Henann D, Franck C. (2014). 3D viscoelastic traction force microscopy. Soft Matter. PMCID: PMC4176508. DOI:10.1039/C4SM01271B

Beane OS, Fonseca VC, Darling EM. (2014). Adipose-derived stem cells retain their regenerative potential after methotrexate treatment. Exp Cell Res. 327 (2014): 222-233. PMCID: PMC4164584DOI:10.1016/j.yexcr.2014.06.015

Desai HV, Voruganti IS, Jayasuriya C, Chen Q, Darling EM. (2014). Live-cell, temporal gene expression analysis of osteogenic differentiation in adipose-derived stem cells. Tissue Eng Pt A. 20(5-6): 899-907. PMCID: PMC3938923DOI: 10.1089/ten.TEA.2013.0761


2013

Athanasiou KA, Darling EM, Duraine GD, Hu JC, Reddi AH. (2013). “Articular Cartilage.” Taylor & Francis Group, LLC: Boca Raton, FL. IBSN: 9781439853245 

Gonzalez-Cruz RD, Darling EM. (2013). Adipose-derived stem cell fate is predicted by cellular mechanical properties. Adipocyte. 2 (2): 87-91. PMCID: PMC3661107. DOI:10.4161/adip.23015

Darling EM, Athanasiou KA. (2013) Bioactive scaffold design for articular cartilage engineering. Biomedical Technology and Devices Handbook. 2nd edition. Ed. by J. Moore and D. Maitland. DOI:10.1201/b15085-23


2012

Beane OS, Darling EM. (2012). Isolation, Characterization, and Differentiation of Stem Cells for Cartilage Regeneration. Ann Biomed Eng. 40 (10) pp. 2079-97. PMCID: PMC3457050. DOI:10.1007/s10439-012-0639-8

Gonzalez-Cruz RD, Fonseca VC, Darling EM. (2012). Cellular mechanical properties reflect differentiation potential of adipose-derived mesenchymal stem cells. Proc Natl Acad Sci USA. 109 (24) E1523-9. PMCID: PMC3386052. DOI:10.1073/pnas.1120349109 

    Desai HV, Darling EM. (2012). Force Scanning for Simultaneous Collection of Topographical and Mechanical Properties. Microscopy and Analysis, January 2012. Volume 26, Issue 1.


    2011

    Darling EM. (2011). Force scanning: A rapid, high-resolution approach for spatial mechanical property mapping. Nanotechnology. 22 (17) 175707. PMCID: PMC3150532. DOI:10.1088/0957-4484/22/17/175707
    *Recognized by Nanotechnology in their “Highlights 2011” Collection.

    Gilchrist CL, Darling EM, Chen J, Setton LA. (2011). Extracellular matrix ligand and stiffness modulate immature nucleus pulposus cell‐cell interactions. PLoS One. 6 (11) e27170. PMCID: PMC3210142. DOI:10.1371/journal.pone.0027170


    2010

    Yim EKF, Darling EM, Kulangara K, Guilak F, Leong KW. (2010). Nanotopography-induced changes in focal adhesions, cytoskeletal organization, and mechanical properties of human mesenchymal stem cells. Biomaterials. 31 (2010) pp. 1299-1306. PMCID: PMC2813896. DOI:10.1016/j.biomaterials.2009.10.037

    Darling EM*, Wilusz RE*, Bolognesi MP, Zauscher S, Guilak F. (2010). Spatial mapping of the biomechanical properties of the pericellular matrix of articular cartilage measured in situ via atomic force microscopy. Biophys J. 98 pp. 2848-2856. PMCID: PMC2884253. DOI:10.1016/j.bpj.2010.03.037 (*equal contributions)


    2009

    Darling EM, Pritchett PE, Evans BA, Superfine R, Zauscher S, Guilak F. (2009). Mechanical properties and gene expression of chondrocytes on micropatterned substrates following dedifferentiation in monolayer. Cell Molec Bioeng. 2 (3) pp. 395-404. PMCID: PMC2898162. DOI:10.1007/s12195-009-0077-3

    Athanasiou KA, Darling EM, Hu JCY. (2009). Articular Cartilage Tissue Engineering. Morgan & Claypool Publishers: San Rafael, CA. DOI:10.2200/S00212ED1V01Y200910TIS003


    2008

    Darling EM, Topel M, Zauscher S, Vail TP, Guilak F. (2008) Viscoelastic properties of human mesenchymally-derived stem cells and primary osteoblasts, chondrocytes, and adipocytes. J Biomech. 41 (2) pp. 454-464. PMCID: PMC2897251. DOI:10.1016/j.jbiomech.2007.06.019

    Coles J, Blum J, Jay G, Darling EM, Guilak F, Zauscher S. (2008) In situ friction measurement on murine cartilage by atomic force microscopy. J Biomech. 41 (3) pp. 541-548. PMCID: PMC2274896. DOI:10.1016/j.jbiomech.2007.10.013

    Darling EM, Guilak F. (2008) A neural network model for cell classification based on single-cell biomechanical properties. Tissue Eng Part A. 14 (9) pp. 1507-1515. PMCID: PMC2748927. DOI:10.1089/ten.tea.2008.0180


    2007

    Darling EM, Zauscher S, Block JA, Guilak F. (2007) A thin-layer model for viscoelastic, stress-relaxation testing of cells using atomic force microscopy:  Do cell properties reflect metastatic potential? Biophys J. 92 pp. 1784-1791. PMCID: PMC1796808. DOI:10.1529/biophysj.106.083097


    2006

    Darling EM, Zauscher S, Guilak F. (2006) Viscoelastic properties of zonal articular chondrocytes measured by atomic force microscopy. Osteoarthritis Cartilage. 14 (6) pp. 571-579. PMID: 16478668. DOI:10.1016/j.joca.2005.12.003


    2005

    Darling EM, Athanasiou KA. (2005) Rapid phenotypic changes in passaged articular chondrocyte subpopulations. J Orthop Res. 23 (2) pp. 425-432. PMID: 15734258. DOI:10.1016/j.orthres.2004.08.008

    Darling EM, Athanasiou KA. (2005) Retaining zonal chondrocyte phenotype by means of novel growth environments. Tissue Eng. 11 (3/4) pp. 395-403. PMID: 15871669. DOI:10.1089/ten.2005.11.395

    Darling EM, Athanasiou KA. (2005) Growth factor impact on articular chondrocyte subpopulations. Cell Tissue Res. 322 pp. 463-473. PMID: 16047167. DOI:10.1007/s00441-005-0020-4


    2004

    Darling EM, Hu JCY, Athanasiou KA. (2004) Zonal and topographical gene expression in articular cartilage. J Orthop Res. 22 (6) pp. 1182-1187. PMID: 15475195. DOI:10.1016/j.orthres.2004.03.001

    Darling EM, Athanasiou KA. (2004) Bioactive scaffold design for articular cartilage engineering. Biomedical Technology and Devices Handbook. Ed. by J. Moore and G. Zouridakis. Chapter 21. CRC Press: Boca Raton, FL. ISBN: 9780849311406


    2003

    Darling EM, Athanasiou KA. Articular cartilage bioreactors and bioprocesses. (2003) Tissue Eng. 9 (1) pp. 9-26. PMID: 12625950. DOI:10.1089/107632703762687492

    Darling EM, Athanasiou KA. (2003) Biomechanical strategies for articular cartilage regeneration. Ann Biomed Eng. 31 pp. 1114-1124. PMID: 14582614. DOI:10.1114/1.1603752