Publications

PRIMO publications:

Pieplow A, Dastaw M, Sakuma T, Sakamoto N, Yamamoto T, Yajima M, Oulhen N, Wessel GM (2021).  CRISPR-Cas9 editing of non-coding genomic loci as a means of controlling gene expression in the sea urchin. Developmental Biology Dev Biol.2021 Jan 19;S0012-1606(21)00015-4. doi: 10.1016/j.ydbio.2021.01.003.

 

Tilahun L, Asrat A, Wessel GM Simachew A (2021). In silico Determination of Nitrogen Metabolism in Microbes from Extreme Conditions using Metagenomics Archives of Microbiology, in press.

 

Perillo M, Oulhen N, Foster S, Spurrell M, Calestani C, Wessel GM, (2020). Regulation of dynamic pigment cell states at single-cell resolution. eLife 9:e60388 DOI:10.7554/eLife.60388. PMCID: PMC7455242

 

Foster S, Oulhen N, Wessel GM (2020). A single cell RNA-seq resource for early sea urchin development. Development. 2020 Sep 11;147(17):dev191528. doi: 10.1242/dev.191528. PMCID:PMC7502599

 

Wessel, G.M., Kiyomoto, M., Shen, T. Yajima, M. Genetic manipulation of the pigment pathway in a sea urchin reveals distinct lineage commitment prior to metamorphosis in the bilateral to radial body plan transition. Scientific Reports 10, 1973 (2020). https://doi.org/10.1038/s41598-020-58584-5 PMCID: PMC7005274

 

Li Y, OmoriA, Flores RL, Satterfield S, Nguyen C, Ota T, Tsurugaya T, Ikuta T, Ikeo K, Kikuchi M, Leong JCK, Reich A, Hao M, Wan W, Dong Y, Ren Y, Zhang S, Zeng T, Uesaka M, Uchida Y, Li X, Shibata TF, Bino T, Ogawa K, Shigenobu S, Kondo M, Wang F, Chen L, Wessel GM, Saiga H, Cameron RA, Livingston B, Bradham C, Wang W, Irie N (2020). Genomic tests of body plan transitions from bilateral to pentameral symmetry in Echinoderms. Communications Biology  Jul 10;3(1):371. doi: 10.1038/s42003-020-1091-1. PMCID: PMC7351957

 

Perillo M, Pagano P, Spurrell M, Arnone I, Wessel GM. (2020). Methodology for Whole Mount and Fluorescent RNA in situ Hybridization in Echinoderms: Single, Double, and Beyond. Methods in Molecular Biology pg 195-216. https://doi.org/10.1007/978-1-0716-0974-3_12

 

Wessel GM, Morita S, Oulhen N. (2020) Somatic cell conversion to a germ cell lineage - a violation or a revelation. Journal of Experimental Zoology B Molecular and Developmental Evolution https://doi.org/10.1002/jez.b.22952.

 

Duncan FE, Schindler K, Schultz RM Blengini C, Stein P, Stricker SA, Wessel GM, Williams CJ (2020). Unscrambling the oocyte and the egg: clarifying terminology of the female gamete in mammals. Mol Hum Reprod. 2020 Oct 6:gaaa066. doi: 10.1093/molehr/gaaa066. Online ahead of print. PMID: 33022047

 

Beyene E, Tefera A, Muleta D, Fantahun S, Wessel GM (2020). Molecular identification and performance evaluation of wild yeasts from different Ethiopian fermented products Journal of Food Science and Technology DOI: 10.1007/s13197-020-04377-7

 

Poon J, Fries A, Wessel GM, Yajima M, (2019) Evolutionary modification of AGS protein contributes to formation of micromeres in sea urchins. Nature Communications 10(1):3779 - 3795. doi: 10.1038/s41467-019-11560-8. PMCID: PMC6706577

 

Clark H, Knapik LO, Zhang Z, Wu X, Naik MT, Oulhen N, Wessel GM, Brayboy LM, (2019). Dysfunctional MDR-1 disrupts mitochondrial homeostasis in the oocyte and ovary. Scientific Reports Jul 3;9(1):9616. doi: 10.1038/s41598-019-4602 PMCID: PMC6610133

 

 

Gebreslassie E, Tefera A, Muleta D, Kiros S, Wessel GM (2019). Optimization of the Cultivation Conditions of Indigenous Wild Yeasts and Evaluation of their Leavening Capacity. International Journal of Science. Vol 8: 15-23. doi: 10.18483/ijSci.2043 

 

Oulhen N, Swartz SZ, Wang L, Wikramanayake A, Wessel GM. (2019). Distinct transcriptional regulation of Nanos2 in the germ line and soma by the Wnt and delta/notch pathways. Dev Biol. 452(1):34-42. doi: 10.1016/j.ydbio.2019.04.010. PMCID: PMC6848975

 

Foster S+, Teo YV+, Neretti N, Oulhen N, Wessel GM (2019). Single cell RNA-seq in the sea urchin embryo shows marked cell-type specificity in the Delta/Notch pathway. Molecular Reproduction and Development Aug;86(8):931-934. doi: 10.1002/mrd.23181. Epub 2019 Jun 14. (+ co-first authors). PMCID: PMC6690749

 

Vecchioni S, Capece MC, Toomey E, Le N, Ray A, Greenberg A, Fujishima K, Urbina J, Paulino-Lima I, Pinheiro V, Shih J, Wessel GM, Wind S, Rothschild LJ. (2019). Construction and characterization of metal ion-containing DNA nanowires for synthetic biology and nanotechnology. Scientific Reports volume 9, Article number: 6942. https://doi.org/10.1038/s41598-019-43316-1 PMCID: PMC6502794

 

Roux-Osovitz M, Foltz KR, Oulhen N, Wessel GM (2019).  Trapping, Tagging and Tracking: Tools for the Study of Proteins During Early Development of the Sea Urchin. Methods in Cell Biology 151:283-304. doi: 10.1016/bs.mcb.2018.11.003. PMCID: PMC7549693

 

Oulhen N. Foster S, Wray G, Wessel GM (2019). Identifying gene expression from single cells to single genes. Methods in Cell Biology 151:127-158. doi: 10.1016/bs.mcb.2018.11.018. PMCID: PMC7384399

 

Campanale J, Hamdoun A, Wessel GM, Su Y-H, Oulhen N (2019). Methods to label, isolate, and image sea urchin small micromeres. Methods in Cell Biology 150:269-292. doi: 10.1016/bs.mcb.2018.11.007. PMCID: PMC6487853

 

Lin C-Y, Oulhen N, Wessel GM, Su Y-H (2019). CRISPR/Cas9-mediated genome editing in sea urchins. Methods in Cell Biology 151:305-321. doi: 10.1016/bs.mcb.2018.10.004. PMCID: PMC7357425

 

Fresques T, Wessel GM (2018). Nodal induces sequential restriction of germ cell factors during primordial germ cell specification. Development Jan 22;145(2). pii: dev155663. doi: 10.1242/dev.155663. PMCID:PMC5825842

 

Wessel, G. M. (2018). Echinodermata. In M. K. Skinner (Ed.), Encyclopedia of Reproduction. vol. 6, pp. 533–545. Academic Press: Elsevier. http://dx.doi.org/10.1016/B978-0-12-809633-8.20600-6

 

Calestani C, Wessel GM (2018). These Colors Don’t Run: Regulation of Pigment Biosynthesis in Echinoderms. Marine Organisms as Model Systems in Biology and Medicine. Kloc M, and Kubiak J. Editors SpringerNature. 2018;65:515-525. doi: 10.1007/978-3-319-92486-1_22. PMID:30083933

 

Brayboy LM Clark H, Schnirman R, Knapik L, Wessel GM (2018). Nitrogen mustard exposure perturbs oocyte mitochondrial physiology and alters reproductive outcomes.  Reproductive Toxicology Oct 9;82:80-87. PMID 30308227. doi: 10.1016/j.reprotox.2018.10.002. PMCID: PMC7050192

 

Tilahun B, Bahiru A, Tesfaye A, Muleta D, Terefework Z, and Wessel GM (2019) Molecular Characterization of fermenting Yeast Species from Fermenting Teff Dough during preparation of Injera using ITS DNA Sequence. International Journal of Food Science, Volume 2019, Article ID 1291863, 7 pages https://doi.org/10.1155/2019/1291863

 

Brayboy LM, Knapik LO, Long S, Westrick M, Wessel GM. (2018). Ovarian hormones modulate multidrug resistance transporters in the ovary. Contraception and Reprod Med. 3:26. PMID: 30460040; doi: 10.1186/s40834-018-0076-7. PMCID: PMC6236903

 

Tilahun B, Tesfaye A, Muleta D, Terefework Z, Bahiru A, Wessel G (2018) Isolation and Molecular Identification of lactic acid Bacteria using 16s rDNA from Fermented Teff (Eragrostis teff (Zucc.))  Dough.  International Journal of Food Science, Volume 2019, Article ID 1291863, 7 pages https://doi.org/10.1155/2019/1291863

 

Oulhen N, Wessel GM (2017). A quiet space during rush hour: Quiescence in primordial germ cells. Stem Cell Research 25: 296-299. PMCID: PMC5732843

 

Niikura K, Alam S, Naruse M, Jimbo M, Moriyama H, Reich A, Wessel GM, Matsumoto M (2017). Protein Kinase A Activity Leads to the Extension of the Acrosomal Process in Starfish Sperm Molecular Reproduction and Development Jul;84(7):614-625. doi: 10.1002/mrd.22824. Epub 2017 Jun 19.

 

Schudrowitz N, Takagi S, Wessel G, Yajima M. (2017). The germline factor DDX4 functions in blood-derived cancer cell phenotypes. Cancer Science Aug;108(8):1612-1619. doi: 10.1111/cas.13299. Epub 2017 Jul 11.

 

Shevidi S, Uchida A, Schudrowitz N, Wessel GM, Yajima M (2017). Single nucleotide editing without DNA cleavage using CRISPR/Cas9-deaminase in the sea urchin embryo. Developmental Dynamics 246:1036-1046. DOI: 10.1002/dvdy.24586 PMCID: PMC5872153

 

Bucci C, Francoeur M, McGreal J, Smolowitz R, Zazueta-Novoa V, Wessel GM, Gomez-Chiarri M. (2017) Sea Star Wasting Disease in Asterias forbesi along the Atlantic Coast of North America. PLoS ONE 12(12):e0188523. https://doi.org/ 10.1371/journal.pone.0188523 PMCID:PMC5724889


Oulhen N, Swartz Z, Laird J, Mascaro A, Wessel GM (2017). Transient Translational Quiescence in Primordial Germ Cells.  Development 144, 1201-1210 doi:10.1242/dev.144170

 

Brayboy LM, Oulhen N,  Long S, Voigt N, Raker C, Wessel GM (2017).Multidrug Resistance Transporter-1 and Breast Cancer Resistance Protein protect against ovarian toxicity, and are essential in ovarian physiology. Reproductive Toxicology 69: 121-131. http://dx.doi.org/10.1016/j.reprotox.2017.02.002

 

Oulhen N, Wessel GM (2016). Albinism as a visual, in vivo guide for CRISPR/Cas9 functionality in the sea urchin embryo. Molecular Reproduction and Development 2016 Dec;83(12):1046-1047. doi: 10.1002/mrd.22757.

 

Oulhen N, Heyland A, Carrier T, Zazueta-Novoa V, Fresques T, Laird J, Onorato T, Janies D, Wessel GM (2016). Regeneration in bipinnaria larvae of the bat star Patiria miniata induces rapid and broad new gene expression. Mechanisms of Development 142: 10-21. doi: 10.1016/j.mod.2016.08.003.

 

Oulhen N, Wessel GM (2016). Differential Nanos 2 protein stability results in selective germ cell accumulation in the sea urchin. Developmental Biology  418: 146-156 doi: 10.1016/j.ydbio.2016.07.007 PMCID: PMC5031544

 

Zazueta-Novoa, V, Onorato T, Luis Reyes Chavez G, Oulhen N, Wessel GM (2016) The complexity of yolk proteins and their dynamics in the sea star, Patiria miniata. Biological Bulletin Jun;230 (3):209-19. PMCID: PMC5103698 DOI: 10.1086/BBLv230n3p209

 

Fresques T, Swartz Z, Juliano C, Morino Y, Kikuchi M, Akasaka K, Wada H, Yajima M, Wessel GM (2016). The Diversity of Nanos Expression in Echinoderm Embryos Supports Different Mechanisms in Germ Cell Specification. Evolution & Development Jul;18(4):267-78. DOI:10.1111/ede.12197 PMCID: PMC4943673

 

Poon J, Wessel GM, Yajima M (2016).An unregulated regulator: Vasa expression in the development of somatic cells and in tumorigenesis. Developmental Biology 415: 24-32. doi:10.1016/j.ydbio.2016.05.012

 

Wessel GM (2016).Germ line mechanics – and unfinished business. In “Essays of Developmental Biology” (Ed. P. Wassarman) Current Topics in Developmental Biology Volume 117:553-66. doi: 10.1016/bs.ctdb.2015.11.030. Epub 2016 Jan 7. Elsevier Press, Amsterdam

 

Dresselhaus T, Sprunck S, Wessel GM (2016). Fertilization mechanisms in flowering plants. Current Biology 26(3):R125-39. doi: 10.1016/j.cub.2015.12.032.

 

Brayboy LM, Wessel GM (2016). The double-edged sword of the mammalian oocyte – advantages, drawbacks, and approaches for basic and clinical single cell analysis. Molecular Human Reproduction 22(4):311-318. doi:10.1093/molehr/gaw020 PMCID:PMC4767051

 

Reich A, Dunn C, Akasaka K, Wessel GM. (2015).Phylogenetic Analyses of Echinodermata Support the Sister Groups of Asterozoa and Echinozoa. PLoS One Mar 20; 10(3);e0119627. Doi:10.1371/journal.pone.0119627.  PMCID:PMC4368666

 

Yajima M, Wessel GM (2015).Essential elements for translation: the germ line factor, Vasa, functions broadly in somatic cells. Development 142(11):1960-70. doi: 10.1242/dev.118448. PMCID:PMC4460737

 

Swartz SZ, Wessel GM. (2015). Germ Line Versus Soma in the Transition from Egg to Embryo. In “The Maternal-to-Zygotic Transition” (Ed. H. Lipshitz) Current Topics in Developmental Biology V. 113. Elsevier Press, Amsterdam. Chapter 32.

 

Kelel M, Abera G Yisma A, Molla B, Gebre N, Adugna T, Wessel G (2014). Isolation of phosphate solubilizing bacteria from Acacia tree rhizosphere soil. Journal of Microbiology and Biotechnology Research 4: 9-13. 

 

Angione S, Oulhen N, Brayboy L, Tripathi A, Wessel GM (2014). Simple Perfusion Apparatus (SPA) for Manipulation, Tracking, and Study of Oocytes and Embryos.  Fertility and Sterility 103(1):281-290.e5. doi: 10.1016/j.fertnstert.2014.09.039. PMCID:PMC4768872

 

Ramos I, Wessel G. (2014). Two-pore Channels (TPCs) function in calcium regulation in sea star oocytes and embryos. Development 141(23):4598-609. doi: 10.1242/dev.113563. PMCID: PMC4302940

 

Kelel, M, Temam S, Mandefro A, Asfaw F, Wessel GM (2014). Bovine Tuberculosis Prevalence in Slaughtered Cattle at Gondar Elfora Abattoir, Based on Meat Inspection and Microscopic Methods. American-Eurasian Journal of Scientific Research 9 (5): 114-119.

 

Swartz SZ, Reich AM, Oulhen N, Raz T, Milos PM, Campanale JP, Hamdoun A, Wessel GM. (2014). Deadenylase depletion protects inherited mRNAs in primordial germ cells. Development 141(16):3134-42. doi: 10.1242/dev.110395. PMCID:PMC4197533

 

Zazueta V, Wessel GM (2014). Protein degradation machinery is present broadly during early development in the sea urchin. Gene Expression Patterns 15(2):135-41. doi: 10.1016/j.gep.2014.06.002. PMCID: PMC4125525

 

Yewhalaw D, Kelel M, Getu E, Temam S, Wessel G. (2014). Blood meal sources and sporozoite rates of Anophelines in Gilgel-Gibe dam area, Southwestern Ethiopia. African Journal of Vector Biology September 2014

 

Oulhen N, Xu D, Wessel GM (2014). Conservation of sequence and function in fertilization of the cortical granule serine protease in echinoderms. BBRC 450: 1135-1141. PMCID:PMC4133295

 

Campanela J, Oulhen N, Wessel G, Hamdoun A (2014).  Migration of sea urchin primordial germ cells. Developmental Dynamics 243: 917-27.  PMCID: PMC4164171

 

Oulhen N, Onorato T, Ramos I, Wessel GM (2014). Dysferlin is essential for endocytosis in the sea star oocyte. Developmental Biology 388: 94-102. DOI information: 10.1016/j.ydbio.2013.12.018. PMCID: PMC3982204

 

Yajima M, Gustafson EA, Song JL, Wessel GM (2014). Piwi regulates Vasa accumulation during embryogenesis in the sea urchin. Developmental Dynamics 243:451–458. PMCID: PMC4018429

 

Juliano CE, Reich A, Liu N, Götzfried J, Zhon M, Uman S, Reenan, R, Wessel GM, Steele RE, Lin H (2014). PIWI-piRNA pathway function in Hydra somatic stem cells Proceedings of the National Academy of Sciences 111, no. 1 (2014): 337–342. doi:10.1073/pnas.1320965111 PMCID: PMC3890812