Characterize the transcriptional and epigenetic networks that control FRT TRM identity. Our phenotypic characterization has revealed a significant heterogeneity among the TRM populations. We hypothesize that FRT TRM are transcriptionally diverse and their transcriptional heterogeneity is driven by differential chromatin accessibility. This hypothesis will be tested by performing computational integration of single cell RNA-seq (scRNA-seq) and assay for transposase accessible chromatin sequencing (ATAC-seq) to identify key molecular regulators that control the differentiation trajectories of these distinct populations. This will be further correlated with the functional potential of TRM in the event of pathogenic infection. We aim to generate a comprehensive transcriptional and epigenetic map of anti-pathogenic mucosal TRM.