Mouse Transgenic and Gene Targeting Facility

Welcome to the Mouse Transgenic and Gene Targeting Facility (MTGTF) at Brown University!

The primary role of the MTGTF is to support the investigators in using genetically modified mouse models in Brown University, affiliated hospitals and academic institutions in Rhode Island and other states. The MTGTF provides services of molecular design and generation of transgenic and knock-out mouse models as well as general advice on the use and management of such models.  The cutting-edge technologies of the CRISPR/Cas9 system are well established in the MTGTF to accomplish vast majority of the projects in a way of cost friendly, fast, and high quality. The conventional ES cell gene-targeting system is employed to serve as an alternative or to fill the limitations of the CRISPR/Cas9 system. Routine services include genotype analysis, sperm or embryo cryopreservation and storage, rederivation, in vitro fertilization (IVF). Other services, such as mouse vasectomy, embryo transfer, colony scale-up,  intracytoplasmic sperm injection (ICSI) are also available. New services requiring MTGTF resources can be created through a request. 

The Transgenic Facility is supported by NIH funding. The following acknowledgement must appear in any publication made possible by the work of the Transgenic Facility:
"This project was supported by a grant from the National Institute of General Medical Sciences (P30 GM103410) from the National Institutes of Health."