Cellular proteomics using mass cytometry has emerged as a key analytic tool for cancer biology as well as clinical samples. A liquid sample containing cells labeled with heavy metal isotope conjugated probes is introduced into the nebulizer where it is aerosolized. The aerosol droplets are vaporized, and ionized and processed so that a cloud of ions enters a time-of-flight (TOF) chamber where the probes are separated based on mass-to-charge ratio as they accelerate towards the detector. The time-resolved detector measures a mass spectrum that represents the identity and quantity of each isotopic probe on a per cell basis.
Advise investigators with experimental design, implementation and data analysis. Provide support for grant submissions.
Director: Patrycja Dubielecka-Szczerba, PhD
Assistant Professor, Brown University
Email: [email protected]