Fast-forward a couple of decades, and genome sequencing technology has now caught up with Rand’s original hypothesis — and it’s validated the earlier work. Last month, the newest study from Rand’s research team was published in the Proceedings of the National Academy of Sciences. Led by Brown graduate student Joaquin Nunez, the team reported molecular evidence of natural selection on the Mpi gene in Semibalanus balanoides, confirming the hypothesis that the gene helps the barnacle to survive the extreme changes of the intertidal zone.

Nunez, who joined Rand’s lab nearly five years ago, analyzed the genomic data to tie it together.

“You always build upon the knowledge that’s before you,” Nunez said.

The team showed, at the nucleotide level, that different versions of the Mpi gene are present at different frequencies depending where the barnacles are found in the intertidal zone. The enzyme plays an important role in glycolysis, which converts sugars to energy; one form performs well under high stress, like a hot day at low tide; the other form does better under low stress.

The study shows how natural selection enables the barnacles’ survival in this ever-changing environment by maintaining multiple versions of the gene that codes for that enzyme.

The paper also uncovered more information about the structural impact of the gene variant. Nunez found new positions in the protein that the gene encodes, where mutations altered the sequence of the protein and, presumably, its function. Colleagues from Sweden modeled the two versions of the protein, suggesting how they would conform to different environmental stressors. These predicted structures are consistent with the differing Mpi enzyme response to its environment.

Future research, Rand said, could use structural biology to “help us elucidate the structures of those alternative forms and get more of the mechanistic details of how this enzyme works.”

Nunez said they also found hints that Mpi may interact with other proteins, producing new characteristics in stressful events — another potential area of study. Though this paper focuses on the adaptations of this barnacle species, the bigger picture is the biological system in which the barnacle operates, he said.

“A very complex set of things needs to work together for these populations to be healthy and thrive for millennia,” Nunez said. “One of the big predictions of climate change is that many natural populations are going to find themselves in environments that aren’t just hotter or more humid or more stressful, but those environments are going to experience very strong fluctuations.”

The intertidal is a “natural laboratory” to understand how species survive and prosper in fluctuating extremes, Nunez said. The same methods that he and Rand used to show how barnacle genes adapt to those extremes could be used by entomologists who want to know how honeybees are adapting to climate change, or crop scientists concerned about productivity in a more stressful environment.

“There are labs all around the world looking for evolutionary approaches to solve this adaptation to climate change,” Rand said.

In addition to Rand and Nunez, other authors from Brown were Patrick Flight, Kimberly Neil, Stephen Rong and David Ferranti. Leif Eriksson, Magnus Alm Rosenblad and Anders Blomberg from the University of Gothenburg also co-authored the paper.

The National Science Foundation (IGERT: DGE-0966060), the National Institutes of Health (2R01GM067862), the Carl Trygger Foundation (CTS 11:14), and the Swedish Research Council (2017-04559, 2014-03914) supported the research. Nunez, Neil and Rong are all NSF Graduate Research Fellows and IGERT Fellows, and Ferranti was supported by a Brown University UTRA Fellowship.