An overview of methods for moving files onto and off of Oscar. Topics covered include: Linux command line tools for file transfer (scp, rsync, sftp), GUI-based file transfer applications, mounting Oscar’s filesystem using CIFS, and using Globus on Oscar.

This will be a virtual workshop. Registered participants will receive an email with instructions for connecting via Zoom the day of the workshop.

Drop in on the Zoom meeting to ask members of CCV’s High-Performance Computing (HPC) team your questions about using Oscar or any other research computing topics you are interested in.

This workshop is for people who are already familiar with Slurm, but would like to use Slurm’s more powerful features. Topics covered include: dependencies for conditional execution of jobs, job arrays for parameter sweeps, dealing with hundreds or thousands of small tasks, how to limit the number of jobs running at once, and how to cancel multiple jobs.

This will be a virtual workshop. Registered participants will receive an email with instructions for connecting via Zoom the day of the workshop.

Drop in on the Zoom meeting to ask members of CCV’s High-Performance Computing (HPC) team your questions about using Oscar or any other research computing topics you are interested in.

This workshop is for people who are already familiar with Slurm, but would like to use Slurm’s more powerful features. Topics covered include: dependencies for conditional execution of jobs, job arrays for parameter sweeps, dealing with hundreds or thousands of small tasks, how to limit the number of jobs running at once, and how to cancel multiple jobs.

This will be a virtual workshop. Registered participants will receive an email with instructions for connecting via Zoom the day of the workshop.

The next Carney Institute External Postdoc Seminar Series (BrainExPo) will feature Ashley Ingiosi, Ph.D., Postdoctoral Research Associate at Washington State University.

Abstract

Sleep is characterized by dynamic changes in neuronal activity, and waking neuronal activity is thought to increase sleep need. Changes in non-neuronal cells (e.g. glia) across the sleep-wake cycle and their role in sleep regulation are comparatively unexplored. I investigated if glial cells called astrocytes also change dynamically across sleep and wake states and if astroglial signaling mechanisms are important for sleep regulation. Astrocytes are not electrically excitable but use calcium (Ca2+) to mediate signaling. Therefore, Ca2+ imaging is optimal for studying astroglial activity. I quantified astroglial Ca2+ activity during sleep-wake behavior in mice expressing the Ca2+ indicator GCaMP6f selectively in cortical astrocytes. Ca2+ activity was captured in vivo with an epifluorescent miniscope in freely-behaving mice and with two-photon microscopy in unanesthetized, head-restrained mice for more detailed exploration of astroglial processes. I recorded astroglial Ca2+ dynamics simultaneously with sleep-wake behavior under baseline conditions, in response to sleep deprivation, and following genetic depletion of astroglial intracellular Ca2+ stores. I found that 1) astroglial Ca2+ signals change dynamically with sleep, wake, and sleep loss, 2) astroglial Ca2+ activity is more pronounced in processes compared to somata, 3) astroglial Ca2+ is responsive to changes in sleep need, 4) synchrony of astroglial Ca2+ signals changes with vigilance state and sleep loss but does not mirror neuronal electrical activity, and 5) reduced astroglial Ca2+ via knockout of stromal interaction molecule 1 reduces sleep drive after sleep loss. Overall, this research revealed a new level of brain organization in a non-neuronal cell type that changes dynamically with vigilance state and plays a role in sleep regulation. These findings could trigger a fundamental shift in our understanding of sleep and sleep regulation.

Drop in on the Zoom meeting to ask members of CCV’s High-Performance Computing (HPC) team your questions about using Oscar or any other research computing topics you are interested in.

Drop in on the Zoom meeting to ask members of CCV’s High-Performance Computing (HPC) team your questions about using Oscar or any other research computing topics you are interested in.

Drop in on the Zoom meeting to ask members of CCV’s High-Performance Computing (HPC) team your questions about using Oscar or any other research computing topics you are interested in.

Drop in on the Zoom meeting to ask members of CCV’s High-Performance Computing (HPC) team your questions about using Oscar or any other research computing topics you are interested in.

Drop in on the Zoom meeting to ask members of CCV’s High-Performance Computing (HPC) team your questions about using Oscar or any other research computing topics you are interested in.

Drop in on the Zoom meeting to ask members of CCV’s High-Performance Computing (HPC) team your questions about using Oscar or any other research computing topics you are interested in.

Drop in on the Zoom meeting to ask members of CCV’s High-Performance Computing (HPC) team your questions about using Oscar or any other research computing topics you are interested in.

Drop in on the Zoom meeting to ask members of CCV’s High-Performance Computing (HPC) team your questions about using Oscar or any other research computing topics you are interested in.

Drop in on the Zoom meeting to ask members of CCV’s High-Performance Computing (HPC) team your questions about using Oscar or any other research computing topics you are interested in.