BL-OG is a neuroscience research technique that combines BioLuminescence with OptoGenetics. Bioluminescence is light created by a chemical reaction within or near a living thing and is released when an enzyme (luciferase) oxidizes a small molecule (luciferin). Optogenetic molecules are those with an element (an opsin) that is sensitive to light. In BL-OG, the enzyme is near the optogenetic element (e.g., an ion channel). When the small molecule is added, it is broken down by the enzyme to produce bioluminescent light that, in turn, activates the optogenetic element. When BL-OG is used in neurons, it can activate or silence their activity and can cause changes in behavior. Further, the bioluminescent light produced serves as a visual indicator that the small molecule reached its enzyme target. In other words, BL-OG can be used to control neurons and behavior, and researchers can see light emitted from their tissue of interest when it is activated or silenced. Learn more. 

Think this tool could work with your research? Start by reading our E-Z Guide to LMO3. You can also order our LMO3 BioLuminescence-OptoGenetics (BL-OG) mouse from Jackson Labs. Try your own behavior, development, nervous system repair or other research question. For help getting started, email [email protected].